INTRODUCTION : Dengue is a Vector borne Disease which is caused by a virus and transmitted by Aedes mosquitoes. A Geographic Information System (GIS) was used as an analytical tool to study the spatial distribution of dengue virus infection among Aedes mosquitos in Tamilnadu. Databases are available with village wise code. The dengue infection among field caught Aedes female mosquito were subjected through reverse transcriptase - polymerase chain reaction (RTPCR) assay and its description in GIS mapping of Tamilnadu was the main motto of this study. In laboratory experiments, all assays showed sufficient sensitively to detect viraemia among the infected mosquito through RT-PCR. The mosquito pools for this study w ere collected from all the Health unit districts including urban areas and segregated into positive and negative pools affected villages respectively and the same has been depicted in GIS mapping so as to study the area or village with positive case incidence as well as positive in mosquito pools. Thus, the Geographic distribution of the virus infected Aedes mosquitos, village wise locations were depicted in GIS l inked with the human cases. Test results out of 13220 Aedes mosquito pools collected, 514 places found positive along with dengue cases incidence are depicted and linked in GIS mapping and there is a linkage with positive correlation between dengue positive and mosquito pool positive ( r = 0.3443,) The development of a village wise RT-PCR laboratory-based dengue virus infection among Aedes mosquitoes with the reporting of dengue cases c an successfully serve as an epidemiological tools in an early warning signal for dengue epidemics and simultaneously initiate the control measures in anticipation.